MirGeneDB 3.0
MirGeneDB ID

Mgi-Mir-2-o58a

Family name MIR-2 (all species)
Species Pacific oyster (Magallana gigas)
MiRBase ID
Paralogues Mgi-Mir-2-o56  Mgi-Mir-2-o57  Mgi-Mir-2-o58b  Mgi-Mir-2-o59  Mgi-Mir-2-o60  Mgi-Mir-2-o61  Mgi-Mir-2-o62  Mgi-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) C. gigas
Node of Origin (family) Protostomia
Genome context
(CGI_GCA_000297895.1)		 scaffold1503: 15162-15221 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o58a)		 Mir-2-o62 scaffold1503: 13873-13931 [-] Ensembl
Mir-2-o58a scaffold1503: 15162-15221 [-] Ensembl
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
GAUGACCAGAAUUCUUUGAUGCGUGUCUGUCAGUCAAAGCUGCUGUGAACUGUGACCAGAAAAUUCAGAUCACAGCACGCUUUGAUGGCUGGAACUGCAUUGUCUUCAUACAUGUCUGGA
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Structure
        10          20         30        40         50         
GAUGACCAGAAUUCUUU--      UG   -|   G       -        A   UGACCA 
                   GAUGCG  UCU GUCA UCAAAGC UGCUGUGA CUG      \
                   UUACGU  AGG CGGU AGUUUCG ACGACACU GAC      G
AGGUCUGUACAUACUUCUG      CA   U^   -       C        A   UUAAAA 
.       110       100        90         80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsUG at 5p(-14)
Tissue expression
- +
A- D- D- F- L- Ea Eg Fe Gi Ha He Ju La Ma Ma Ma Pe Re Sp Um
Star sequence

Mgi-Mir-2-o58a_5p*
mirBase accessionNone
Sequence 
0- CAGUCAAAGCUGCUGUGAACUG -22
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Mature sequence

Mgi-Mir-2-o58a_3p
mirBase accessionNone
Sequence 
37- GAUCACAGCACGCUUUGAUGGCU -60
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