MirGeneDB 3.0
MirGeneDB ID

Gsa-Mir-2-o161b

Family name MIR-2 (all species)
Species Russian doll killer (Gyrodactylus salaris)
MiRBase ID gsa-mir-2a
Paralogues Gsa-Mir-2-o161a  Gsa-Mir-2-o162  Gsa-Mir-2-o163 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) G. salaris
Node of Origin (family) Protostomia
Genome context
(gyrodactylus_salaris.PRJNA244375.WBPS19.genomic)		 scf7180006951238: 8098-8159 [+] UCSC Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o161b)		 Mir-71-o7 scf7180006951238: 7887-7948 [+] UCSC Ensembl
Mir-2-o161a scf7180006951238: 8098-8159 [+] UCSC Ensembl
Mir-2-o161b scf7180006951238: 8098-8159 [+] UCSC Ensembl
Mir-2-o162 scf7180006951238: 8224-8285 [+] UCSC Ensembl
Seed AUCACAG
Precursor
(pre-Mir +30nt flank)		 
CAUUGAACUACAGUCCCAUCUAUUAGGACCGUCCGAGUGCGACUGUGAUGUUAUGUCAUGUUUGCAAUAUCACAGUCCUGCUUUGGUGACGGUGCAAUGAUGGUAUACAGCACCGCUGUAUU
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Structure
        10        20          30           40        50         
CAUUGAACUACAGUCCCAU      GG--      C---|    C            AUGUCA 
                   CUAUUA    ACCGUC    GAGUG GACUGUGAUGUU      \
                   GGUAGU    UGGCAG    UUCGU CUGACACUAUAA      U
UUAUGUCGCCACGACAUAU      AACG      UGGU^    C            CGUUUG 
120       110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
- +
To
Star sequence

Gsa-Mir-2-o161b_5p*
mirBase accessionMIMAT0035266
Sequence 
0- GUCCGAGUGCGACUGUGAUGUU -22
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Mature sequence

Gsa-Mir-2-o161b_3p
mirBase accessionMIMAT0035267
Sequence 
37- UAUCACAGUCCUGCUUUGGUGACGG -62
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